- Laboratory of Innate Immunity
- Research Theme
- Elucidating mechanisms of innate immune responses in mucosal immunity and pathogenesis of inflammatory bowel disease and lifestyle diseases
- Research Keywords
mucosal immunology, innate immunity, antimicrobial peptides, alpha-defensin, intestinal microbiota, intestinal environment, Paneth cell, stem cell, inflammatory bowel disease, lifestyle diseases, gastroenterology, food and medicine, priventive medicine, therapeutics
Overview of Research
Paradigm shift for the mucosal immune system:
Through both biological and medical insights, we test mechanisms in the innate enteric immunity both microbial elimination and symbiosis with the intestinal microbiota with molecular and cellular levels, and beyond. We will make paradigm shift in the mucosal immunity from viewpoint of the host-microbe interaction.
Elucidating roles of antimicrobial peptides in the innate immunity:
We will clarify pathogenesis of inflammatory bowel disease (Crohn’s disease and ulcerative colitis) and lifestyle diseases, based on innate immune functions of antimicrobial peptides, especially alpha-defensin secreted by Paneth cells in the small intestine. We have been studying Paneth cell functions in the innate enteric immunity, regeneration, and symbiosis. Elucidating mechanisms for action of alpha-defensin from structure-activity relationship and interaction with the intestinal bacteria will contribute to solving global health problems.
Understanding the intestinal environment created with foods, intestinal epithelial cells and intestinal microbiota:
We conduct researches to understand various intestinal functions such as absorption, metabolism, immunity, and brain-gut axis from the molecular and cellular perspectives. We create unique intestinal environment evaluation methods that is compatible with biological functions in vivo using the latest intestinal epithelial cell isolation, culture and imaging technologies and contribute to the development of functional foods and new therapeutics.
- School of Science:
Biological Science course (Macromolecular Functions), Core Laboratories
- Graduate School of Life Science:
Division of Life Science, Transdisciplinary Life Science Course, Functional Cellular Sciences
Kamioka M, Goto Y, Nakamura K, Yokoi Y, Sugimoto R, Ohira S, Kurashima Y, Umemoto S, Sato S, Kunisawa J, Takahashi Y, Domino SE, Renauld JC, Nakae S, Iwakura Y, Ernst PB, Ayabe T, Kiyono H. Intestinal commensal microbiota and cytokines regulate Fut2+ Paneth cells for gut defense. Proc Natl Acad Sci U.S.A. 2022. doi: 10.1073/pnas.2115230119.
Shimizu Y, Nakamura K, Kikuchi M, Ukawa S, Nakamura K, Okada E, Imae A, Nakagawa T, Yamamura R, Tamakoshi A, Ayabe T. Lower human defensin 5 in elderly people compared to middle-aged is associated with differences in the intestinal microbiota composition: the DOSANCO Health . Study. GeroScience 2021 doi: 10.1007/s11357-021-00398-y.
Suzuki K, Nakamura K, Shimizu Y, Yokoi Y, Hagiwara M, Ohira S, Wang Y, Song Y, Aizawa T, Ayabe T. Decrease of alpha-defensin impairs intestinal metabolite homeostasis via dysbiosis in mouse chronic social defeat stress model. Sci Rep 2021. doi: 10.1038/s41598-021-89308-y.
Nakamura K, Yokoi Y, Fukaya R, Ohira S, Shinozaki R, Nishida T, Kikuchi M, Ayabe T. Expression and localization of Paneth cells and their alpha-defensins in the small intestine of adult mouse. Front Immunol 2020. doi: 10.3389/fimmu.2020.570296.
Shimizu Y, Nakamura K, Yoshii A, Yokoi Y, Kikuchi M, Shinozaki R, Nakamura S, Ohira S, Sugimoto R, Ayabe T. Paneth cell alpha-defensin misfolding correlates with dysbiosis and ileitis in Crohn’s disease model mice. Life Science Alliance 2020. doi: 10.26508/lsa.201900592.
Yokoi Y, Nakamura K, Yoneda T, Kikuchi M, Sugimoto R, Shimizu Y, Ayabe T. Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids. Sci Rep 2019. doi.org/10.1038/s41598-019-39610-7.
Refer to HOKKAIDO UNIVERSITY RESEARCHERS DIRECTORY
– Time: Anytime during the lecture period
– Place: Frontier-AMLS, 4F
Please contact in advance by E-mail.
<Related SDGs> 3. GOOD HEALTH AND WELL-BEING
4. QUALITY EDUCATION
9. INDUSTRY, INNOVATION AND INFRASTRUCTURE
17. PARTNERSHIPS FOR THE GOALS
- Faculty of Advanced Life Science, Department of Functional Life Sciences, Cell Biological Science
- Frontier Research Center for Advanced Material and Life Science, Academia-Industry Collaboration Unit