Staff

KINJO MasatakaProfessor

Laboratory
Laboratory of Molecular Cell Dynamics
Research Theme
Study of Living Cell using Single Molecular Detcion ad Molecular Interaction
Research Keywords

Fluorescence measurement, Bioimaging, Fluorescence Correlation Spectroscopy, Total Internal Reflection, Biomolecular Interaction, Protein Community

Overview of Research

My main interesting is system of molecular interaction and recognition between biomolecules. Besides the development of single molecular techniques such as fluorescence correlation spectroscopy (FCS), fluorescence cross correlation spectroscopy (FCCS) and other techniques, we aim at the quantitative analysis of protein dynamics and interactions in living cell using FCS and FCCS. Hopefully, single molecule detection and related techniques have enabled us to clarify how the biomolecues can make cell function in whole body system by the molecular interaction.

However, we recognized life could not be understood from only DNA sequences. Life is not only associated by sum of mechanism of molecular machines.

To understand of life and life system, we have to clarify not only the total number of biomolecule but also dynamics and interactions between biomolecular network. The characteristic biomolecular network makes life and individuals. To analyze such biomolecular network, besides the understanding function of each molecular machine, we have to clarify the interaction among biomolecules as well as how the each molecule could effect to whole function of life in the view point of system biology. To realize such our aim, we will carry out from wide range of single molecule detection to live cell imaging.

Charge

Representative Publications

Aleksandar J. Krmpot, Stanko N. Nikolić, Sho Oasa, Dimitrios K. Papadopoulos, Marco Vitali, Makoto Oura, Shintaro Mikuni, Per Thyberg, Simone Tisa, Masataka Kinjo, Lennart Nilsson, Lars Terenius, Rudolf Rigler, and Vladana Vukojević., Functional Fluorescence Microscopy Imaging: Quantitative Scanning-Free Confocal Fluorescence Microscopy for the Characterization of Fast Dynamic Processes in Live Cells, Anal. Chem 91(17), 11129-11137(2019)

Yamamoto J. and Kinjo M., Full fiber-optic fluorescence correlation spectroscopy, Optics Express, 27(10), 14835-14841 (2019)

Sugihara M, Morito D, Ainuki S, Hirano Y, Ogino K, Kitamura A, Hirata H, Nagata K, The AAA+ ATPase/ubiquitin ligase mysterin stabilizes cytoplasmic lipid droplets, J Cell Biol. 218(3), 949-960(2019)

Takahashi S., Yamamoto J., Kitamura A., Kinjo M.,Sugimoto N., Characterization of Intracellular Crowding Environments with Topology-Based DNA Quadruplex Sensors, Anal. Chem., 91(4), pp 2586–2590 (2019)

Kitamura A., Kinjo M., State-of-the-art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation., Int. J. Mol. Sci., 19, 964, (2018)

 

Refer to HOKKAIDO UNIVERSITY RESEARCHERS DIRECTORY

(https://researchers.general.hokudai.ac.jp/profile/en.roAdxRX6Sl-bZBl0k9xszQ==.html)

(https://researchers.general.hokudai.ac.jp/profile/en.e2599dda45f8a29d520e17560c007669.html)

Note

<Office Hour>
– Time: Anytime during the lecture period
– Place: Frontier-AMLS, 6F
Please contact in advance by E-mail.
E-mail: kinjo[at]sci.hokudai.ac.jp

Affiliation