Laboratory of Biological Information Analysis Science

Elucidation of the molecular mechanisms of photoactive proteins
Research Theme
Elucidation of the light-energy conversion mechanisms of photoactive proteins / Development of photoactive systems by featuring functionally modified photoactive proteins
Research Keywords

photoactive proteins, photobiology, rhodopsin, membrane protein, ion pump, membrane transport, transporter, NMR, biomimicry


Overview of Research and Education

Organisms express photoactive proteins to utilize sunlight for cellular metabolic processes. Rhodopsins are the most ubiquitous photoactive proteins and have diverse functions, such as photosensors, ion pumps, and ion channels. We are analyzing their essential mechanisms for light-energy utilization and functional differentiation. Our goal is to develop the novel photoactive proteins applicable to environmental and medical problems.

Laboratory of Biological Information Analysis Science (pdf)



N10, W8, Kita-ku, Sapporo, Hokkaido
demura* (Please replace * with @ when sending e-mail.)

Representative Publications

Keisuke Murabe, Takashi Tsukamoto, Tomoyasu Aizawa, Makoto Demura, Takashi Kikukawa, Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin, J. Am. Chem. Soc. 142, 16023-16030 (2020)

Azusa Iizuka, Kousuke Kajimoto, Tomotsumi Fujisawa, Takashi Tsukamoto, Tomoyasu Aizawa, Naoki Kamo, Kwang-Hwan Jung, Masashi Unno, Makoto Demura, Takashi Kikukawa, Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin, Scientific Reports, 9:10711 (2019)

Takatoshi Hasemi, Takashi Kikukawaa, Yumi Watanabe, Tomoyasu Aizawa, Seiji Miyauchi, Naoki Kamo, and Makoto Demura, Photochemical study of a cyanobacterial chloride-ion pumping rhodopsin, Biochim. Biophys. Acta – Bioenergetics, 1860, 136-146 (2019)

Takashi Tsukamoto, Chihiro Kikuchi, Hiromu Suzuki, Tomoyasu Aizawa,Takashi Kikukawa & Makoto Demura, Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl− concentration, Scientific Reports, 8:13445 (2018)

Shintaro Nakamura, Takashi Kikukawa, Jun Tamogami, Masakatsu Kamiya, Tomoyasu Aizawa, Martin W Hahn, Kunio Ihara, Naoki Kamo, Makoto Demura, Photochemical characterization of actinorhodopsin and its functional existence in the natural host, Biochim. Biophys. Acta – Bioenergetics, 1857, 1900-1908 (2016)

Takatoshi Hasemi, Takashi Kikukawa, Naoki Kamo, Makoto Demura, Characterization of a Cyanobacterial Chloride-Pumping Rhodopsin and Its Conversion into a Proton Pump, J. Biol. Chem., 291, 355-362 (2016)