Laboratory

Laboratory of Molecular Cell Dynamics

Protein homeostasis (proteostasis) elucidating from biomolecular dynamics
Research Theme
Protein condensation and aggregation / Protein homeostasis (proteostasis) / Spectroscopic imaging methods
Research Keywords

Proteostasis, Protein aggregation, Neurodegenerative disease, C. elegans, Fluorescence Correlation Spectroscopy, Fluorescence imaging techniques, Single molecule analysis, Molecular interaction

Staff

Overview of Research and Education

Research Contents: Biological system is the typical example of the general concept of supramolecule, where the numerous functions are originated from the complex interactions among the biologically active molecules, cells, tissues and organs. As for specific biological issues, we would like to promote research to determine the cause of neuronal cell death caused by protein aggregates/condensates associated with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and glaucoma, and to elucidate the cytoprotective role played by non-membrane organelle aggregates. In addition to cultured cell lines, phenotypic and lifespan analysis of C. elegans is also conducted.

Laboratory of Molecular Cell Dynamics (pdf)

Charge

Contact

Address
〒001-0021
Kita-21 Nishi-11 Kita-ku, Sapporo, Hokkaido
Phone
011-706-9006
Fax
011-706-9045
Email
fcs2007*sci.hokudai.ac.jp (Please replace * with @ when sending e-mail.)

Representative Publications

Fujimoto A., Lyu Y., Kinjo M., Kitamura A., Interaction between spike protein of SARS-CoV-2 and human virus receptor ACE2 using two-color fluorescence cross-correlation spectroscopy., Applied Sciences, 11, 10697, 2021

Kitamura A., Numazawa R., Kinjo M., Conformational stabilization of Optineurin by the dynamic interaction of linear polyubiquitin., Biochem. Biophys. Res. Commun., 559, 203-209, 2021

Yang G., Hiruma S., Kitamura A., Kinjo M., Mishra, M., Uehara R., Molecular basis of functional compatibility between ezrin and other actin-membrane associated proteins during cytokinesis., Exp. Cell Res., 403, 112600, 2021

Harusawa K., Watanabe C., Kobori Y., Tomita K., Kitamura A., Kinjo M., Yanagisawa M., Membrane surface modulates slow diffusion in small crowded droplets., Langmuir, 37, 437-444, 2021

Takakura H., Goto Y., Kitamura A., Yoshihara T., Tobita S., Kinjo M., Ogawa M., Analysis of the Triplet-state Kinetics of a Photosensitizer for Photoimmunotherapy by Fluorescence Correlation Spectroscopy., J. Photochem. & Photobiol., A: Chem., 408, 113094, 2021