Staff

NAKAMURA KiminoriAssociate Professor

Laboratory
Laboratory of Innate Immunity
Research Theme
The effect of oral commensal bacteria on intestinal innate immunity
Research Keywords

Mucosal immunology, Innate immunity, Antimicrobial peptides, Defensin, Intestine, Paneth cell, Stem cell, Infectious disease, Inflammatory bowel disease, Regenerative medicine

Overview of Research

It is thought that oral commensal bacteria influence the intestinal bacterial flora and innate immunity, since the oral cavity and the bowels are anatomically consecutive. However, a detailed examination of the influence of the oral cavity resident flora on bowel function has not yet been conducted. One reason is the lack of an appropriate intestinal epithelial cell line that can be used in vitro. Therefore, we aim to establish a method by which to culture mouse small intestine epithelial cells and to establish a cell line.
By using the obtained cells, we analyze the innate immune system of the bowel and the crosstalk between the epithelial cells and the oral commensal bacteria.
The number of patients with Inflammatory Bowel Disease (IBD) has been increasing continuously on a world-wide basis, including in Japan. Finally, to understand pathophysiology and pathogenesis of IBD, Crohn’s disease and ulcerative colitis in particular, we analyze the roles for AMPs, related signal molecules in Paneth cells as well as crosstalk between innate and adaptive immunity in the gut. Taken together, we are eager to contribute to improving the health of patients with IBD and infectious diseases by improving our knowledge of AMPs as effectors of innate intestinal immunity and by developing new therapeutics.

Charge

Message

If you have the motivation and idea, you have an environment where you can freely proceed with your research. We hope motivated students interested in innate immunity and intestinal microbiota to join our lab.

Representative Publications

Nakamura K, Yokoi Y, Fukaya R, Ohira S, Shinozaki R, Nishida T, Kikuchi M, Ayabe T. Expression and localization of Paneth cells and their α-defensins in the small intestine of adult mouse. Front Immunol 2020. 11: 570296. doi: 10.3389/fimmu.2020.570296.

Shimizu Y, Nakamura K, Yoshii A, Yokoi Y, Kikuchi M, Shinozaki R, Nakamura S, Ohira S, Sugimoto R, Ayabe T. Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn’s disease model mice. Life Science Alliance 2020. doi:10.26508/lsa.201900592.

Yokoi Y, Nakamura K, Yoneda T, Kikuchi M, Sugimoto R, Shimizu Y, Ayabe T. Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids. Sci Rep 2019. doi.org/10.1038/s41598-019-39610-7.

Takakuwa A, Nakamura K, Kikuchi M, Sugimoto R, Ohira S, Yokoi Y, Ayabe T. Butyric acid and leucine induce alpha-defensin secretion from small intestinal Paneth cells. Nutrients 2019, 11(11), 2817; doi.org/10.3390/nu11112817.

Eriguchi Y, Nakamura K, Yokoi Y, Takahashi S, Hashimoto D, Teshima T, Ayabe T, Selsted ME, Ouellette AJ. Essential role of interferon-gamma in T cell-associated intestinal inflammation. JCI Insight 2018. doi: 10.1172/jci.insight.121886.

Hayase E, Hashimoto D, Nakamura K, Noizat C, Ogasawara R, Takahashi S, Ohigashi H, Yokoi Y, Sugimoto R, Matsuoka S, Ara T, Yokoyama E, Yamakawa T, Ebata K, Kondo T, Hiramine R, Aizawa T, Ogura Y, Hayashi T, Mori H, Kurokawa K, Tomizuka K, Ayabe T, Teshima T., R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease., J Exp Med 2017. 214: 3507-3518.

Refer to HOKKAIDO UNIVERSITY RESEARCHERS DIRECTORY
(https://researchers.general.hokudai.ac.jp/profile/en.P5or0wmgKDGd6T1oJuMusg==.html)

Note

<Office Hour>
– Time: Anytime during the lecture period
– Place: Frontier-AMLS, 4F
Please contact in advance by E-mail.
E-mail: kiminori[at]sci.hokudai.ac.jp

<Related SDGs>  
3. GOOD HEALTH AND WELL-BEING
4. QUALITY EDUCATION
9. INDUSTRY, INNOVATION AND INFRASTRUCTURE
17. PARTNERSHIPS FOR THE GOALS

Affiliation