Laboratory of Biomolecular Adaptation Science

Research Theme
Elucidation and application of molecular functions exhibited by cold-responsive proteins / Development of functional nucleic acids and application to oligonucleotide therapeutics / Research on microorganism adaptation to cold conditions Analysis and improvement of protein functions for industrial application
Research Keywords

Antifreeze protein, Ice-binding function, Snow mold fungi, Gene expression, Biomoleculart NMR, X-ray structural analysis, Biodiversity, Cell-preservation peptide, Reporter Assay, 3D molecular structure, Gene detection, Nucleic acid drugs, Functional DNA/RNA, Molecular detection, Cell-function evaluation, Electrochemistry


Overview of Research and Education

Research Contents:

Laboratory of Biomolecular Adaptation Science(s.tsuda2018)



AIST-Hokkaido, 2-17-2-1 Tsukisamu-Higashi, Toyohira, Sapporo
s.tsuda* (Please replace * with @ when sending e-mail.)

Representative Publications

Rahman, AT., Arai, T., Yamauchi, A., Miura, A., Kondo, H., Ohyama, Y., and Tsuda, S.: Ice recrystallization is strongly inhibited when antifreeze proteins bind to multiple ice planes. Sci. Rep.(2019), 9, 1, 2212.

Arai, T., Fukami, D., Hoshino, T., Kondo, H., and Tsuda, S.: Ice-binding proteins from the fungus Antarctomyces psychrotropicus possibly originate from two different bacteria through horizontal gene transfer. FEBS J. (2019) 286, 5, 946-962.

Azuma, N., Miyazaki, Y., Nakano, M., and Tsuda, S.: Unexpected rise of glass transition temperature of ice crystallized from antifreeze protein solution. J. Phys. Chem. Lett., 9, 4512-4515 (2018).

Mahatabuddin, S., Fukami, D., Arai,T., Nishimiya, Y., Shimizu, R., Shibazaki, C., Kondo, H., Adachi, M., and Tsuda, S.: Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein. Proc. Natl. Acad. Sci. USA,115 (21), 5456-5461 (2018).

Mahatabuddin, S., Hanada, Y., Nishimiya, Y., Miura, A., Kondo, H., Davies, PL., and Tsuda, S. Concentration-dependent oligomerization of an alpha-helical antifreeze polypeptide makes it hyperactive. Sci. Rep.(2017), 7, 42501.