TSUDA SakaeVisiting Professor

Visiting faculty
Research Theme
Functional Analysis of Low-temperature-adapted Protein and Its Applications
Research Keywords

Antifreeze Protein, AFP, Ice-nucleation protein, INP, Thermal Hysteresis, Ice-growth-inhibition, NMR, X-ray, Hydration, water structure

Overview of Research

A main interest of S.Tsuda is to clarify mechanism of a new functional protein by utilizing the advanced techniques of molecular biology, biochemistry, and structural biology (NMR and X-ray). His current targets are the antifreeze proteins (fish AFPI~III, fish AFGP, and fungi hyperactive AFP), which have originally been discoveredd from Japanese organisms in the last 10 years.


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  • Graduate School of Life Science:


S. Tsuda has been operating the HU laboratory of Biomolecular Adaptation since 2003, and moved to Graduate school of frontier sciences, the university of Tokyo in 2022 April. He now studies a macromolecule named “Antifreeze Protein” by employing many advanced techniques of physicochemistry and molecular biology, which will realize an advanced technique that contributes to SDGs.

Representative Publications

Okamoto R, Orii R, Shibata H, Maki Y, Tsuda S, and Kajihara Y. : Regulating Antifreeze Activity through Water: Latent Functions of the Sugars of Antifreeze Glycoprotein Revealed by Total Chemical Synthesis. Chem-Eur J. e202203553. doi: 10.1002/chem.202203553, (2023).

Arai, T., Yamauchi, A., Yang, Y., Singh, S.M., Sasaki, Y.C., and Tsuda, S. : Adsorption of ice-binding protein onto whole ice crystal surfaces does not confer a high thermal hysteresis activity. Sci. Rep. 12, 15443, doi: 10.1038/s41598-022-19803-3, (2022).

Arai, T., Fukami, D., Hoshino, T., Kondo, H., and Tsuda, S. : Ice-binding proteins from the fungus Antarctomyces psychrotropicus possibly originate from two different bacteria through horizontal gene transfer. FEBS J., 286, 5, 946-962, doi: 10.1111/febs.14725(2019).

Azuma, N., Miyazaki, Y., Nakano, M., and Tsuda, S. : Unexpected rise of glass transition temperature of ice crystallized from antifreeze protein solution. J. Phys. Chem. Lett., 9, 4512-4515, doi: 10.1021/acs.jpclett.8b01492 (2018).

Mahatabuddin, S., Fukami, D., Arai,T., Nishimiya, Y., Shimizu, R., Shibazaki, C., Kondo, H., Adachi, M., and Tsuda, S. : Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein. Proc. Natl. Acad. Sci. USA, 115 (21), 5456-5461, doi: 10.1073/pnas.1800635115 (2018).


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