TSUDA SakaeVisiting Professor
- Laboratory
- Visiting faculty
- Research Theme
- Functional Analysis of Low-temperature-adapted Protein and Its Applications
- Research Keywords
Antifreeze Protein, AFP, Ice-nucleation protein, INP, Thermal Hysteresis, Ice-growth-inhibition, NMR, X-ray, Hydration, water structure
Overview of Research
A main interest of S.Tsuda is to clarify mechanism of a new functional protein by utilizing the advanced techniques of molecular biology, biochemistry, and structural biology (NMR and X-ray). His current targets are the antifreeze proteins (fish AFPI~III, fish AFGP, and fungi hyperactive AFP), which have originally been discoveredd from Japanese organisms in the last 10 years.
Charge
- School of Science:
No - Graduate School of Life Science:
No
Representative Publications
Okamoto R, Orii R, Shibata H, Maki Y, Tsuda S, and Kajihara Y. : Regulating Antifreeze Activity through Water: Latent Functions of the Sugars of Antifreeze Glycoprotein Revealed by Total Chemical Synthesis. Chem-Eur J. e202203553. doi: 10.1002/chem.202203553, (2023).
Arai, T., Yamauchi, A., Yang, Y., Singh, S.M., Sasaki, Y.C., and Tsuda, S. : Adsorption of ice-binding protein onto whole ice crystal surfaces does not confer a high thermal hysteresis activity. Sci. Rep. 12, 15443, doi: 10.1038/s41598-022-19803-3, (2022).
Arai, T., Fukami, D., Hoshino, T., Kondo, H., and Tsuda, S. : Ice-binding proteins from the fungus Antarctomyces psychrotropicus possibly originate from two different bacteria through horizontal gene transfer. FEBS J., 286, 5, 946-962, doi: 10.1111/febs.14725(2019).
Azuma, N., Miyazaki, Y., Nakano, M., and Tsuda, S. : Unexpected rise of glass transition temperature of ice crystallized from antifreeze protein solution. J. Phys. Chem. Lett., 9, 4512-4515, doi: 10.1021/acs.jpclett.8b01492 (2018).
Mahatabuddin, S., Fukami, D., Arai,T., Nishimiya, Y., Shimizu, R., Shibazaki, C., Kondo, H., Adachi, M., and Tsuda, S. : Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein. Proc. Natl. Acad. Sci. USA, 115 (21), 5456-5461, doi: 10.1073/pnas.1800635115 (2018).
Note
<Correspondence>
– E-mail: s-tsuda@edu.k.u-tokyo.ac.jp
– Website:https://www.sakaetsuda.jp/index_e.html
Affiliation
- Faculty of Advanced Life Science, visiting faculty