TSUDA SakaeVisiting Professor

Laboratory of Biomolecular Adaptation Science
Research Theme
Functional Analysis of Low-temperature-adapted Protein and Its Applications
Research Keywords

Antifreeze Protein, AFP, Ice-nucleation protein, INP, Thermal Hysteresis, Ice-growth-inhibition, NMR, X-ray, Hydration, water structure

Overview of Research

A main interest of S.Tsuda is to clarify mechanism of a new functional protein by utilizing the advanced techniques of molecular biology, biochemistry, and structural biology (NMR and X-ray). My current targets are the antifreeze proteins (fish AFPI~III, fish AFGP, and fungi hyperactive AFP), which have originally been explored from Japanese organisms in the last 10 years.


  • School of Science:
  • Graduate School of Life Science:
    Division of Life Science, Transdisciplinary Life Science Course, Biomolecular Adaptation Science (Inter-field Cooperation with AIST)


The professors (Tsuda & Komatsu) and assoc. professors (Kondo & Hirano) of this laboratory are the staff members of AIST. The principle mission of AIST is to obtain the result that is now actually desired by many people. Hence, the basic motivation of our research is “practical use” of our findings and developments. We while believe that they should be only realized after performing huge basic studies from molecular to industrial level, so that we employ a wide range of advanced techniques from molecular biology to structural biology (NMR and X-Ray). We believe that you are able to have knowledge about how pure science leads to the practical/commercial use. Please write me ( if you have an interest to work with us !

Representative Publications

Yamauchi, A., Arai, T., Kondo, H., Sasaki, Y.C., and Tsuda, S.: An ice-binding protein from an Antarctic ascomycete is fine-tuned to bind to specific water molecules located in the prosm planes. Biomolecules, 10, 759 (2020).

Arai, T., Fukami, D., Hoshino, T., Kondo, H., and Tsuda, S.: Ice-binding proteins from the fungus Antarctomyces psychrotropicus possibly originate from two different bacteria through horizontal gene transfer. FEBS J., 286, 5, 946-962 (2019).

Azuma, N., Miyazaki, Y., Nakano, M., and Tsuda, S.: Unexpected rise of glass transition temperature of ice crystallized from antifreeze protein solution. J. Phys. Chem. Lett., 9, 4512-4515 (2018).

Mahatabuddin, S., Fukami, D., Arai,T., Nishimiya, Y., Shimizu, R., Shibazaki, C., Kondo, H., Adachi, M., and Tsuda, S.: Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein. Proc. Natl. Acad. Sci. USA,115 (21), 5456-5461 (2018).

Mahatabuddin, S., Hanada, Y., Nishimiya, Y., Miura, A., Kondo, H., Davies, PL., and Tsuda, S. Concentration-dependent oligomerization of an alpha-helical antifreeze polypeptide makes it hyperactive. Sci. Rep., 7, 42501 (2017).


<Office Hour>
– Time: Anytime during the lecture period
– Place: Science Bldg. No.2, 8F, #2-804
Please contact in advance by E-mail.
E-mail: s.tsuda[at]


  • National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido