YAMAZAKI TomohikoVisiting Associate Professor

Laboratory of Frontier Biomaterials Science
Research Theme
Bio-nanomolecules for Diagnostic and Therapy Applications
Research Keywords

Protein, Peptide, Oligodeoxynucleotide, Artificial recognition element, Toll like receptor, Adjuvant, G-quadruplex, Hemozoin

Overview of Research

We are developing novel bio-nanomolecules such as oligonucleotide-, peptide-, and protein-based materials, with knowledge gained from the life sciences. We seek to utilize the novel bio-nanomolecules to diagnostic and therapy of cancer, infection and immunological disease.
Toll-like receptor 9 (TLR9) is one of innate immunity receptors recognizing single-stranded DNA derived from viruses and microorganisms, and hemozoin which is malaria-derived heme crystals. The ligands of TLR9 act as a molecule that immuno-activator or suppressor. We elucidated the interaction between TLR 9 and the natural ligand molecule, then developed novel ligand with superior function to natural ligand molecules.


  • School of Science:
  • Graduate School of Life Science:
    Division of Life Science, Transdisciplinary Life Science Course, Frontier Biomaterials Science (Inter-field Cooperation with NIMS)


In our research, integration of scientific knowledge and technique of various fields, such as molecular biology, cell biology, protein engineering, protein function analysis, and also a biomaterial, is indispensable. We offer a supportive environment for promoting your research. Anyone who is interested in our project, please contact us.

Representative Publications

Anh Thi Tram Tu, Kazuaki Hoshi, Miwako Shobo, Tomohiko Yamazaki. G-Quadruplex-Based CpG Oligodeoxynucleotide/DOTAP Complex Strongly Stimulates Immunity in CpG Motif-Specific and Loop-Length-Dependent Manners. Nanomedicine: Nanotechnology, Biology and Medicine 40 (2022) 102508

Fika Ayu Safitri, Anh Thi Tram Tu, Kazuaki Hoshi, Miwako Shobo, Arief Budi Witarto, Sony Heru Sumarsono, Ernawati Arifin Giri-Rachman, Kaori Tsukakoshi, Kazunori Ikebukuro, Tomohiko Yamazaki. Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparallel Guanine-Quadruplex Structural Scaffold. Biomolecules 11 [11] (2021) 1617

Anh Thi Tram Tu, Kazuaki Hoshi, Kazunori Ikebukuro, Nobutaka Hanagata, Tomohiko Yamazaki. Monomeric G-Quadruplex-Based CpG Oligodeoxynucleotides as Potent Toll-Like Receptor 9 Agonists. Biomacromolecules. 21 [9] (2020) 3644-3657

Akane Sakaguchi-Mikami, Kazuhiro Fujimoto, Tetsushi Taguchi, Karube Isao, Tomohiko Yamazaki. A novel biofunctionalizing peptide for metallic alloy. Biotechnology Letters. 42 [5] (2020) 747-756

Satoshi Migita, Kosuke Sakashita, Yuta Saito, Suyalatu, Tomohiko Yamazaki. Co–Cr–Mo alloy binding peptide as molecular glue for constructing biomedical surfaces. Journal of Applied Biomaterials & Functional Materials. 18 (2020) 228080002092473

Kazuaki Hoshi, Tomohiko Yamazaki, Yuuki Sugiyama, Kaori Tsukakoshi, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro. G-Quadruplex Structure Improves the Immunostimulatory Effects of CpG Oligonucleotides. Nucleic Acid Therapeutics. 29 [4] (2019) 224-229

Kazuaki Hoshi, Tomohiko Yamazaki, Chiaki Yoshikawa, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode. Synthesis of a hemin-containing copolymer as a novel immunostimulator that induces IFN-gamma production. International Journal of Nanomedicine. 13 (2018) 4461-4472


Profile on NIMS SAMURAI database


  • National Institute for Materials Science, Research Center for Functional Materials, Nanomedicine Group