Laboratory of Cellular and Molecular Sciences

Protein homeostasis (proteostasis) elucidating from biomolecular dynamics
Research Theme
Protein condensation and aggregation / Protein homeostasis (proteostasis) / Spectroscopic imaging methods
Research Keywords

Proteostasis, Molecular Chaperones, Protein aggregation, Protein folding, Fluorescence measurement, Bioimaging, Fluorescence Correlation Spectroscopy, Biomolecular Interaction, C. elegans


Overview of Research and Education

Research Contents: Biological system is the typical example of the general concept of supramolecule, where the numerous functions are originated from the complex interactions among the biologically active molecules, cells, tissues and organs. As for specific biological issues, we would like to promote research to determine the cause of neuronal cell death caused by protein aggregates/condensates associated with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and glaucoma, and to elucidate the cytoprotective role played by non-membrane organelle aggregates. In addition to cultured cell lines, phenotypic and lifespan analysis of C. elegans is also conducted.

Laboratory of Molecular Cell Dynamics (pdf)



Kita-21 Nishi-11 Kita-ku, Sapporo, Hokkaido
akita* (Please replace * with @ when sending e-mail.)

Representative Publications

Kitamura A., Tornmalm J., Demirbay B., Piguet J., Kinjo M., Widengren J., Trans-cis isomerization kinetics of cyanine dyes reports on the folding states of exogeneous RNA G-quadruplexes in live cells., Nucleic Acids Research, 51(5), 21, 2023

Kitamura A., Yuno S., Kawamura R., Kinjo M., Intracellular conformation of amyotrophic lateral sclerosis-causative TDP-43., International Journal of Molecular Sciences, 24(6), 551322, 2023

Fujimoto A., Lyu Y., Kinjo M., Kitamura A., Interaction between spike protein of SARS-CoV-2 and human virus receptor ACE2 using two-color fluorescence cross-correlation spectroscopy., Applied Sciences, 11, 10697, 2021

Kitamura A., Numazawa R., Kinjo M., Conformational stabilization of Optineurin by the dynamic interaction of linear polyubiquitin., Biochem. Biophys. Res. Commun., 559, 203-209, 2021

Takakura H., Goto Y., Kitamura A., Yoshihara T., Tobita S., Kinjo M., Ogawa M., Analysis of the Triplet-state Kinetics of a Photosensitizer for Photoimmunotherapy by Fluorescence Correlation Spectroscopy., J. Photochem. & Photobiol., A: Chem., 408, 113094, 2021