Laboratory of Molecular Cell Dynamics
- Research Theme
- Protein condensation and aggregation / Protein homeostasis (proteostasis) / Spectroscopic imaging methods
- Research Keywords
Proteostasis, Molecular Chaperones, Protein aggregation, Protein folding, Fluorescence measurement, Bioimaging, Fluorescence Correlation Spectroscopy, Biomolecular Interaction, C. elegans
Staff
Overview of Research and Education
Research Contents: Biological system is the typical example of the general concept of supramolecule, where the numerous functions are originated from the complex interactions among the biologically active molecules, cells, tissues and organs. As for specific biological issues, we would like to promote research to determine the cause of neuronal cell death caused by protein aggregates/condensates associated with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and glaucoma, and to elucidate the cytoprotective role played by non-membrane organelle aggregates. In addition to cultured cell lines, phenotypic and lifespan analysis of C. elegans is also conducted.
Laboratory of Molecular Cell Dynamics (pdf)
Charge
- Charge (US):
School of Science, Biological Science course (Macromolecular Functions), Core Laboratories - Charge (GS):
Graduate School of Life Science, Division of Life Science, Transdisciplinary Life Science Course, Functional Cellular Sciences
Contact
- Address
- 〒001-0021
Kita-21 Nishi-11 Kita-ku, Sapporo, Hokkaido - Phone
- 011-706-9006
- Fax
- 011-706-9045
- fcs2007*sci.hokudai.ac.jp (Please replace ＊ with @ when sending e-mail.)
Representative Publications
Kitamura A., Tornmalm J., Demirbay B., Piguet J., Kinjo M., Widengren J., Trans-cis isomerization kinetics of cyanine dyes reports on the folding states of exogeneous RNA G-quadruplexes in live cells., Nucleic Acids Research, 51(5), 21, 2023
Kitamura A., Yuno S., Kawamura R., Kinjo M., Intracellular conformation of amyotrophic lateral sclerosis-causative TDP-43., International Journal of Molecular Sciences, 24(6), 551322, 2023
Fujimoto A., Lyu Y., Kinjo M., Kitamura A., Interaction between spike protein of SARS-CoV-2 and human virus receptor ACE2 using two-color fluorescence cross-correlation spectroscopy., Applied Sciences, 11, 10697, 2021
Kitamura A., Numazawa R., Kinjo M., Conformational stabilization of Optineurin by the dynamic interaction of linear polyubiquitin., Biochem. Biophys. Res. Commun., 559, 203-209, 2021
Takakura H., Goto Y., Kitamura A., Yoshihara T., Tobita S., Kinjo M., Ogawa M., Analysis of the Triplet-state Kinetics of a Photosensitizer for Photoimmunotherapy by Fluorescence Correlation Spectroscopy., J. Photochem. & Photobiol., A: Chem., 408, 113094, 2021