Laboratory of X-Ray Structural Biology

Research Theme
Structural biological elucidation regarding the functions of proteins and nucleic acid in relation to biological phenomena / Structural and functional study on translational control / Structural biology of the disease-related factors / Method development of X-ray protein crystallography
Research Keywords

Protein, nucleic acid, translational control, signal transduction, infection regulation, disease related factor, X-ray/neutron structural biology, quantum biology, protein crystallography, computer science, protein science, biophysics


Overview of Research and Education

The elucidation of the molecular mechanisms in which converters genetic information into functional molecules like RNAs and proteins is a central subject of molecular biology. We analyze the structure and function of proteins to elucidate their reaction mechanisms and intermolecular network, by using various physical, physicochemical and biochemical methods, such as crystallography, electron microscopy, calorimetry and activity measurement. We also aim at the applications of obtained structural information to drug design and industry.

Laboratory of X-Ray Structural BiologyPDF





5-307, North 10 West 8, Kita-ku, Sapporo

yao* (Please replace * with @ when sending e-mail.)

Representative Publications

Selective Recent Publications

Takehito Tanzawa, Koji Kato, Dylan Girodat, Toyoyuki Ose, Yuki Kumakura, Hans-Joachim Wieden, Toshio Uchiumi, Isao Tanaka, and Min Yao, The C-terminal helix of ribosomal P stalk recognizes a hydrophobic groove of elongation factor 2 in a novel fashion, Nucleic Acids Research. 46, 3232-3244 (2018)

Meirong Chen, Koji Kato, Yume Kubo, Yoshikazu Tanaka, Yuchen Liu, Feng Long, William Whitman, Pascal Lill, Christos Gatsogiannis, Stefan Raunser, Nobutaka Shimizu, Akira Shinoda, Akiyoshi Nakamura, Isao Tanaka, and Min Yao, Structural basis for the tRNA-dependent cysteine biosynthesis, Nature Communications. 8, 1512-1532 (2017)

Shingo Nojima, Ayumi Fujishima, Koji Kato, Kayoko Ohuchi, Nobutaka Shimizu, Kento Yonezawa, Kenji Tajima and Min Yao, Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C, Scientific Reports. 7, 13018 (2017)

Shoko Kimura, Tateki Suzuki, Meirong Chen, Koji Kato, Jian Yu, Akiyoshi Nakamura, Isao Tanaka, Min Yao, Template-dependent nucleotide addition in the reverse (3′-5′) direction by Thg1-like protein, Science Advances. 2, e1501397 (2016)

 Tateki Suzuki, Akiyoshi Nakamura, Koji Kato, Dieter Soll, Isao Tanaka, Kelly Sheppard, and Min Yao, Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis, Proc. Natl. Acad. Sci. USA . 112, 382-387 (2015)