Staff

MONDE KenjiProfessor

Laboratory
Laboratory of Molecular Chemical Biology
Lipid Biofunction Section (Additional Post)
Research Theme
Three-dimensional structural analysis of biological molecules by vibrational circular dichroism (VCD) spectrum / Creation of disease related molecules by lipid chemical biology
Research Keywords

Chemical Biology, Chirality, Vibrational Circular Dichroism, Glycoscience, Lipidscience, Helix, Metabolites

Overview of Research

Molecular chirality is a fundamental property which governs various biological phenomena, and is the source of secondary and higher-order structures of biomacromolecules. Our approach for understanding biological systems is based on a detailed understanding of molecular chiral properties. In contrast to proteins, the conformation and topology of nucleic acids, carbohydrates and lipids have not been extensively studied in current biology. We have applied chiroptical spectroscopies such as vibrational circular dichroism to investigate the chiral structures of various biomolecules, sometimes at an atomic level. Our first goal is to understand and regulate the higher-order structures of biomolecules, and to correlate such structures and their biological functions, by using spectroscopy, organic chemistry and biochemistry.
Our second topics is sphingolipid chemical biology. Sphingolipids constitute a class of lipids defined by their eighteen carbon amino-alcohol backbones. Sphingolipids are one of main membrane components, and play important roles in signal transmission and cell recognition. We are constructing sphingoid lipid chemical library in order to discovery of new inhibitors toward lipid related enzymes. Utilizing theses novel inhibitors, we study for identification of binding mode between ligand/inhibitor and protein through developing novel chemical biological technologies such as photo affinity labelling. Our second goal is developing novel innovative chem-biological tools, which contribute to the lipid chemical biology leading to new type of drugs.

Charge

Representative Publications

Mahadeva M. M. Swamy, Yuta Murai, Kenji Monde, Setsuko Tsuboi, Aravind K. Swamy, Takashi Jin, Biocompatible and water-soluble shortwave-infrared (SWIR) emit-ting cyanine-based fluorescent probes for in vivo multiplexed molecular imaging. ACS Appl. Mater. Interfaces (2024) in press.

Taniguchi, T., Suzuki, T., Satoh, H., Shichibu, Y., Konishi, K., Monde, K., Preparation of Carbodiimides with One-Handed Axial Chirality, J. Am. Chem. Soc., 140, 15577-15581 (2018)

Swamy, M. M. M., Murai, Y., Ohno, Y., Jojima, K., Kihara, A., Mitsutake, S., Igarashi, Y., Yu, J., Yao, M., Suga, Y., Anetai, M., Monde, K., Structure-inspired design of a sphingolipid mimic sphingosine-1-phosphate receptor agonist from a naturally occurring sphingomyelin synthase inhibitor, Chem. Commun., 54, 12758 – 12761, (2018)

Gowda S. B., Nakahashi A., Yamane, K., Nakahashi, S., Murai, Y., C. Siddegowda, A. K., Hammam M. A. S., Monde, K., Facile Chemoselective Strategy toward Capturing Sphingoid Bases by Unique Glutaraldehyde Functionalized Resin, ACS Omega, 3, 753-759 (2018)

Kato, M., Hammam, M. A. S., Taniguchi, T., Suga, Y., Monde, K., What Is the True Structure of D609, a Widely Used Lipid Related Enzyme Inhibitor?, Org. Lett., 18, 768-771 (2016)

Taniguchi, T., Manai, D., Shibata, M., Itabashi, Y., Monde, K., Stereochemical Analysis of Glycerophospholipids by Vibrational Circular Dichroism., J. Am. Chem. Soc., 137, 12191–12194 (2015)

Refer to HOKKAIDO UNIVERSITY RESEARCHERS DIRECTORY
(https://researchers.general.hokudai.ac.jp/profile/en.yRzoHROg092Lc9Ff5h5Zaw==.html)

Note

<Office Hour>
– Time: Anytime during the lecture period
– Place: Frontier-AMLS, 4F
Please contact in advance by E-mail.
E-mail: kmonde[at]sci.hokudai.ac.jp

Affiliation