Laboratory

Laboratory of Protein Science

Protein Sciences, from production to application
Research Theme
Studies on production methods for recombinant proteins and peptides / Research on proteins and peptides using NMR spectroscopy and genetic engineering / NMR metabolomics
Research Keywords

protein science, structural biology, protein engineering, NMR, metabolome, biomarker research, biological defense, innate immunity, antimicrobial peptides, allergen, cytokines, microbiological control

Staff

Overview of Research and Education

Our main research targets are NMR analysis of proteins and peptides, development of novel production methods of heterologous proteins and peptides. Our final goals of the research are to create the artificial proteins with complete control. We also utilize NMR for metabolomics researches. Our group are conducting research on NMR metabolomics which comprehensively analyzes metabolites of living organisms in various body fluids related to health and diseases.

Laboratory of Protein Science(pdf)

Practical Subjects
Bio
Chem
Phy
Math

Charge

Contact

Address
〒060-0810
N10, W8, Kita-ku, Sapporo, Hokkaido
Phone
011-706-3806
Fax
011-706-3806
Email
aizawa*sci.hokudai.ac.jp (Please replace ＊ with @ when sending e-mail.)

Representative Publications

Song Y, Wang Y, Yan S, Nakamura K, Kikukawa T, Ayabe T, Aizawa T., Efficient recombinant production of mouse-derived cryptdin family peptides by a novel facilitation strategy for inclusion body formation. Microb Cell Fact., 22, 9 (2023)

Gu H, Kato T, Kumeta H, Kumaki Y, Tsukamoto T, Kikukawa T, Demura M, Ishida H, Vogel HJ, Aizawa T.,  Three-Dimensional Structure of the Antimicrobial Peptide Cecropin P1 in Dodecylphosphocholine Micelles and the Role of the C-Terminal Residues., ACS Omega., 7, 31924-31934 (2022)

Iizuka T, Takei M, Saito Y, Rumi F, Zheng J, Lu X, Chafey P, Broussard C, Guilloux-Assalet L, Charpin D, Ebisawa M, Sénéchal H, Aizawa T, Poncet P., Gibberellin-regulated protein sensitization in Japanese cedar (Cryptomeria japonica) pollen allergic Japanese cohorts., Allergy., 76, 2297-2302 (2021)

Komatsu Y, Shimizu Y, Yamano M, Kikuchi M, Nakamura K, Ayabe T, Aizawa T., Disease Progression-Associated Alterations in Fecal Metabolites in SAMP1/YitFc Mice, a Crohn’s Disease Model., Metabolomics, 16, 48 (2020)

Sénéchal H, Šantrůček J, Melčová M, Svoboda P, Zídková J, Charpin D, Guilloux L, Shahali Y, Selva MA, Couderc R, Aizawa T, Poncet P., A new allergen family involved in pollen food-associated syndrome: Snakin/gibberellin-regulated proteins., J Allergy Clin Immunol, 141, 411-414 (2018)

Penkhrue W, Sujarit K, Kudo T, Ohkuma M, Masaki K, Aizawa T, Pathom-Aree W, Khanongnuch C, Lumyong S., Amycolatopsis oliviviridis sp. nov., a novel polylactic acid-bioplastic-degrading actinomycete isolated from paddy soil., Int J Syst Evol Microbiol, 68, 1448-1454 (2018)

Hayase E, Hashimoto D, Nakamura K, Noizat C, Ogasawara R, Takahashi S, Ohigashi H, Yokoi Y, Sugimoto R, Matsuoka S, Ara T, Yokoyama E, Yamakawa T, Ebata K, Kondo T, Hiramine R, Aizawa T, Ogura Y, Hayashi T, Mori H, Kurokawa K, Tomizuka K, Ayabe T, Teshima T., R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease., J Exp Med, 214, 3507-3518 (2017)

Baek M, Kamiya M, Kushibiki T, Nakazumi T, Tomisawa S,, Abe C, Kumaki Y, Kikukawa T, Demura M, Kawano K, Aizawa T, Lipopolysaccharide-bound structure of the antimicrobial peptide cecropin P1 determined by nuclear magnetic resonance spectroscopy., J. Peptide Sci, 22, 214-21 (2016)

Ishida H, Nguyen LT, Gopal R, Aizawa T, Vogel HJ, Overexpression of Antimicrobial, Anticancer, Transmembrane Peptides in Escherichia coli through a Calmodulin-Peptide Fusion System., J Am Chem Soc, 138, 11318-26 (2016)

Refer to HOKKAIDO UNIVERSITY RESEARCHERS DIRECTORY
(https://researchers.general.hokudai.ac.jp/profile/en.YW9I3ckXtNcaTh95IUkcHw==.html)