Laboratory of Advanced Chemical Biology
- Research Theme
- Rational drug discovery based on a theory of dynamic epitope and innovative glycotechnology platform
- Research Keywords
Chemical biology, Medicinal chemistry, Glycomedicine, Automated glycosynthesis, Glycomics, Glycotyping, Glycobiology, Biomarker discovery, Dynamic epitope, Glycopeptide, Antibody drug, Glycoconjugates probe, Microarray, Mass Spectrometry
Staff
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HINOU Hiroshi Professor
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NISHIMURA Shin-Ichiro Specially Appointed Professor
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YOKOI Yasuhiro Assistant Professor
Overview of Research and Education
Research Contents: Toward personalized medicine, our goal is to establish a promising strategy for the rational drug discovery system from disease-relevant “dynamic epitopes” based on the specific posttranslational modification of the key glycoproteins. Our new glycotechnology platform, notably glycoblotting-based high throughput glycomics and microarray displaying robust synthetic glycopeptides library, allowed for the development of epitope-defined antibodies showing potent anti-cancer activities.
At the core of our focused library construction technology, we are continuing to innovate molecular-based life technology such as microarrays and absolute quantification technology of specific key structures in life.
Laboratory of Advanced Chemical Biology (pdf)
Charge
- Charge (US):
School of Science, Biological Science course (Macromolecular Functions), Core Laboratories - Charge (GS):
Graduate School of Life Science, Division of Life Science, Transdisciplinary Life Science Course, Biosystem and Biomolecule Control Science
Contact
- Address
- 〒001-0021
N21, W11, Kita-ku, Sapporo, Hokkdido - Phone
- 011-706-9043
- Fax
- 011-706-9042
- shin*sci.hokudai.ac.jp
hinou＊sci.hokudai.ac.jp
(Please replace * with @ when sending e-mail.)
Representative Publications
Villones Lareno L, Ludwig A-K., Kumeta H., Kikuchi S., Ochi R., Aizawa T., Nishimura S-I., Gabius H-J., Hinou H, “Exploring the In situ pairing of human galectins toward synthetic O-mannosylated core M1 glycopeptides of α-dystroglycan”, Sci. Rep. 12, 17800 (2022)
Otaki M., Hirane N., Natsume-Kitatani Y., Nogami Itoh M., Shindo M., Kurebayashi Y., Nishimura S-I., “Mouse tissue glycome atlas 2022 highlights inter-organ variation in major N-glycan profiles”, Sci. Rep. 12, 17804 (2022)
Shiratori K., Yokoi Y., Wakui H., Hirane N., Otaki M., Hinou H., Yoneyama T., Hatakeyama T., Kimura S., Ohyama C., Nishimura Shin-Ichiro, “Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics” RSC Adv. 12, 21385-21393 (2022)
Pablo A. Guillen-Poza, Elena Matilde Sánchez-Fernández, Gerard Artigas, José Manuel García Fernández, Hinou H., Carmen Ortiz Mellet, Nishimura S-I., Fayna Garcia-Martin, “Amplified detection of breast cancer autoantibodies using MUC1-based Tn antigen mimics” J. Med. Chem. 63, 8524-8533 (2020)
Koide R., Hirane N., Kambe D., Yokoi Y., Otaki M., Nishimura S-I., “Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis” Biomaterials 280, 121314
Yokoi Y., Nishimura S-I. “Effect of Site‐specific O-Glycosylation on the Structural Behavior of NOTCH1 Receptor Extracellular EGF‐like Domains 11 and 10” Chem. Eur. J. 26, 12363-12372 (2020)
Wakui H., Tanaka Y., Ose T., Matsumoto I., Kato K., Yao M., Tachibana T., Sato M., Naruchi K., García-Martín F., Hinou H., Nishimura S-I. “A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes” Chem. Sci. 11, 4999-5006 (2020)
Hayakawa A., Matsushita T., Yokoi Y., Wakui H., Fayna Garcia-Martin, Hinou H., Matsuoka K., Nouso H., Kamiyama T., Taketomi A, Nishimura S-I. “Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes” Biochemistry 59, 1221-1241 (2020)
Koide R., Nishimura S-I., “Antiadhesive nanosomes facilitate targeting of lysosomal GlcNAc salvage pathway through derailed cancer endocytosis” Angew. Chem. Int. Ed. 58, 14513-14518 (2019)
Somovilla V.J., Bermeijo I.A., Albuquerque I.S., Martínez-S. N., Castro-Lopez J., Garcia-Martin F., Companon I.., Hinou H., Nishimura S-I., Jimenez-Barbero J., Asensio J.L, Avenoza G., Busto J.H., Hurtado-Guerrero. R., Peregrina J.M., Bernardes G.J.L., Corzana F., “The use of fluoroproline in MUC1 antigen enables efficient detection of antibodies in patients with prostate cancer”, J. Am. Chem. Soc. 129, 18255-18261 (2017)
Ohyabu N, Kakiya K., Yokoi Y., Hinou H., Nishimura S-I., “Convergent solid-phase synthesis of macromolecular MUC1 models truly mimicking serum glycoprotein biomarkers of interstitial lung diseases”, J. Am. Chem. Soc. 138, 8392-8395(2016)