Laboratory

Laboratory of Biological Information Analysis Science

Understanding the molecular mechanisms of proteins and developing useful proteins
Research Theme
Elucidation of the molecular mechanism of photoreceptor proteins / Functional modification of photoreceptor proteins for useful applications
Research Keywords

Photoactive proteins, photobiology, rhodopsin, optogenetics, laboratory evolution, membrane protein, ion pump, membrane transport, transporter, flash photolysis

Staff

Overview of Research and Education

Organisms express photoactive proteins to utilize sunlight for cellular metabolic processes. Rhodopsins are the most ubiquitous photoactive proteins and have diverse functions, such as photosensors, ion pumps, and ion channels. We are analyzing their essential mechanisms for light-energy utilization and functional differentiation. Our goal is to develop the novel photoactive proteins applicable to environmental and medical problems.

Laboratory of Biological Information Analysis Science (pdf)

Charge

Contact

Address
〒060-0810
N10, W8, Kita-ku, Sapporo, Hokkaido
Phone
011-706-2771
Fax
011-706-2771
Email
demura*sci.hokudai.ac.jp (Please replace * with @ when sending e-mail.)

Representative Publications

Doi, Y., Watanabe, J., Nii, R., Tsukamoto, T., Demura, M., Sudo, Y., Kikukawa, T., Mutations conferring SO42− pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant, Sci. Rep. 12, 16422 (2022)

Sasaki, S., Tamogami, J., Nishiya, K., Demura, M., Kikukawa, T., Replaceability of Schiff base proton donors in light-driven proton pump rhodopsins, J. Biol. Chem. 297, 101013 (2021)

Kato, T., Tsukamoto, T., Demura, M., Kikukawa, T., Real-time identification of two substrate-binding intermediates for the light-driven sodium pump rhodopsin, J. Biol. Chem. 296, 100792 (2021)

Kikuchi, C., Kurane, H., Watanabe, T., Demura, M., Kikukawa, T., Tsukamoto, T., Preference of Proteomonas sulcata anion channelrhodopsin for NO3− revealed using a pH electrode method, Sci. Rep. 11, 7908 (2021)

Murabe, K., Tsukamoto, T., Aizawa, T., Demura, M., Kikukawa, T., Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin, J. Am. Chem. Soc. 142: 16023-16030 (2020)

Iizuka, A., Kajimoto, K., Fujisawa, T., Tsukamoto, T., Aizawa, T., Kamo, N., Jung, KH, Unno, M., Demura, M., Kikukawa, T., Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin, Sci. Rep. 9: 10711 (2019)

Tsukamoto, T., Kikuchi, C., Suzuki, H., Aizawa, T., Kikukawa, T., Demura, M., Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl- concentrations, Sci. Rep., 8: 13445 (2018)

Hasemi, T., Kikukawa, T., Kamo, N., Demura, M., Characterization of a Cyanobacterial Chloride-Pumping Rhodopsin and its Conversion into a Proton Pump, J. Biol. Chem. 291: 355-362 (2016)

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