Laboratory

Laboratory of Cell Dynamics

Cells, the Final Frontier in Science
Research Theme
Collective cell migration in 3D morphogenesis Cancer metastasis Structures and physical properties of polymeric materials and connective tissues
Research Keywords

Cell Mechanics, Collective Cell Behavior, 3D Morphogenesis, Cancer Invasion, Atomic Force Microscopy

Staff

Overview of Research and Education

Research Contents: We are interested in the behaviors of cells and tissues, especially collective cell migration, 3D morphogenesis, and tissue construction. To understand these phenomena, we utilize soft biomaterials (e.g. collagen gels, Matrigels) for cell culture to mimic biological environments and analyze the behaviors with approaches of both molecular biology and mechanobiology. Furthermore, we are using extracellular matrices of different stiffness and investigating the mechanism of cancer progression induced by substrate stiffening. We are able to contribute to improve regenerative medicine and cancer therapy with our research approaches.

Laboratory of Cell Dynamics(PDF

Charge

Contact

Address
〒060-0810
N10, W8, Kita-ku, Sapporo, Hokkaido
Phone
011-706-2769
Fax
011-706-2769
Email
haga*sci.hokudai.ac.jp (Please replace*with @ when sending e-mail.)

Representative Publications

Y. Kumagai, J. Nio-Kobayashi, S. Ishida-Ishihara, H. Tachibana, R. Omori, A. Enomoto, S. Ishihara, and H. Haga: The intercellular expression of type-XVII collagen, laminin-332, and integrin-β1 promote contact following during the collective invasion of a cancer cell population, Biochem. Biophys. Res. Commun., 514, 1115-1121 (2019).

S. Ishihara, K. Aoki, T. Mizutani, M. Amano, S. Nishimura, and H. Haga: Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells, Cell Structure and Function, 43, 177-185 (2018).

Koh, K. Furusawa, and H. Haga: Anisotropic Multi-channel Collagen Gel (MCCG) Guides the Growth Direction of the Neurite-like Processes of PC12 Cells, Scientific Reports, 8, 13901, 1-10 (2018).

Oyama, K. Takahashi, Y. Tanaka, H. Takemoto, and H. Haga: Long-term Culture of Human iPS Cell-derived Telencephalic Neuron Aggregates on Collagen Gel, Cell Structure and Function, 43, 85-94 (2018).

Akiyama, T. Sushida, S. Ishida, and H. Haga: Conformational Mathematical Model of Collective Cell Migrations Based on Cell Polarity, Dev. Growth Differ., 59, 471-490 (2017).

Nukuda, H. Endoh, M. Yasuda, T. Mizutani, K. Kawabata, and H. Haga: Role of ATF5 in the Invasive Potential of Diverse Human Cancer Cell Lines, Biochem. Biophys. Res. Commun., 474, 509–514 (2016).

M. Imai, K. Furusawa, T. Mizutani, K. Kawabata, and H. Haga: Three-dimensional Morphogenesis of MDCK Cells Induced by Cellular Contractile Forces on a Viscous Substrate, Scientific Reports, 5, 14208, 1-10 (2015).

Ishihara, M. Yasuda, A. Ishizu, M. Ishikawa, H. Shirato, and H. Haga: Activating Transcription Factor 5 Enhances Radioresistance and Malignancy in Cancer Cells, Oncotarget, 6, 4602-4614 (2015).

Yamaguchi, T. Mizutani, K. Kawabata, and H. Haga: Leader Cells Regulate Collective Cell Migration via Rac Activation in the Downstream Signaling of Integrin Beta1 and PI3K, Scientific Reports, 5, 7656, 1-8 (2015).

Refer to HOKKAIDO UNIVERSITY RESEARCHERS DIRECTORY
(https://researchers.general.hokudai.ac.jp/profile/en.K6kRFVurrafZ9RWNSKrWFw==.html)