Laboratory of Cell Dynamics
- Research Theme
- Collective cell migration in 3D morphogenesis Cancer metastasis Structures and physical properties of polymeric materials and connective tissues
- Research Keywords
Cell Mechanics, Collective Cell Behavior, 3D Morphogenesis, Cancer Invasion, Atomic Force Microscopy
Staff
Overview of Research and Education
Research Contents: We are interested in the behaviors of cells and tissues, especially collective cell migration, 3D morphogenesis, and tissue construction. To understand these phenomena, we utilize soft biomaterials (e.g. collagen gels, Matrigels) for cell culture to mimic biological environments and analyze the behaviors with approaches of both molecular biology and mechanobiology. Furthermore, we are using extracellular matrices of different stiffness and investigating the mechanism of cancer progression induced by substrate stiffening. We are able to contribute to improve regenerative medicine and cancer therapy with our research approaches.
Laboratory of Cell Dynamics（PDF）
Charge
- Charge (US):
School of Science, Biological Science course (Macromolecular Functions), Core Laboratories - Charge (GS):
Graduate School of Life Science, Division of Soft Matter, Soft Matter Biophysics
Contact
- Address
- 〒060-0810
N10, W8, Kita-ku, Sapporo, Hokkaido - Phone
- 011-706-2769
- Fax
- 011-706-2769
- haga*sci.hokudai.ac.jp (Please replace*with @ when sending e-mail.)
Representative Publications
S. Ishihara, A. Enomoto, A. Sakai, T. Iida, S. Tange, N. Kioka, A. Nukuda, A-I. Nagasato, M. Yasuda, T. Tokino, and H. Haga: Stiff Extracellular Matrix Activates the Transcription Factor ATF5 to Promote the Proliferation of Cancer Cells, iScience, 28, 112057 (2025).
K. Onishi, S. Ishihara, M. Takahashi, A. Sakai, A. Enomoto, K. Suzuki, and H. Haga: Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis, FEBS Letters, 597, 643-656 (2023).
S. Ishihara, H. Kurosawa, and H. Haga: Stiffness-Modulation of Collagen Gels by Genipin-Crosslinking for Cell Culture, Gels, 9, 148 (2023).
S. Ishida-Ishihara, R. Takada, K. Furusawa, S. Ishihara, and H. Haga: Improvement of the cell viability of hepatocytes cultured in three-dimensional collagen gels using pump-free perfusion driven by water level difference, Scientific Reports, 12, 20269, 1-11 (2022).
Y. Kumagai, J. Nio-Kobayashi, S. Ishihara, A. Enomoto, M. Akiyama, R. Ichihara, and H. Haga: The Interferon-β/STAT1 Axis Drives the Collective Invasion of Skin Squamous Cell Carcinoma with Sealed Intercellular Spaces, Oncogenesis, 11, 27 (2022).
H. Oyama, A. Nukuda, S. Ishihara, and H. Haga: Soft Surfaces Promote Astrocytic Differentiation of Mouse Embryonic Neural Stem Cells via Dephosphorylation of MRLC in the Absence of Serum, Scientific Reports, 11, 19574, 1-11 (2021).
S. Ishida-Ishihara, M. Akiyama, K. Furusawa, I. Naguro, H. Ryuno, T. Sushida, S. Ishihara, and H. Haga: Osmotic Gradients Induce Stable Dome Morphogenesis on Extracellular Matrix, Journal of Cell Science, 133, jcs243865 (2020).